Sphera chemistry auto analyzer

SPHERA 202: Built to last Random access instrument Reusable cells with wash station 30 reagent positions

SIZE / WEIGHT Height: 49 cm (19.3 in) Weight: 38 kg (83.8 lbs)
Depth: 60 cm (23.6 in)
Width: 62 cm (24.4 in)

POWER SUPPLY 240 / 100 Vac, 50 / 60 Hrz, single phase with ground
Independent on-off switch for refrigerated reagent plate
Fuse compartment / fuses: 2 Amp @ 230 Vac, 4 Amp @ 115 Vac Power consumption: less than 200 VA (external PC excluded) Ground resistance: less than 0.1 Ohm
Leakage current: less than 2.5 mA

SAMPLING ARM 1 sampling needle,110 mm needle stroke
Capacitive liquid level detector Needle shock sensor

DILUTER SYRINGE Long life plunger
Syringe capacity, 368 µL Syringe resolution, 0.14 µL

HYDRAULIC 8 self-priming peristaltic pumps (life 1000 hrs) SYSTEM with replaceable neoprene cassette (life 500 hrs)
2 vacuum pumps Pinch valve Manifold
Containers*: Water, 20L; Cleaning solution, 2L; Waste, 20L
* Equipped with level sensor and safety connections

WASH STATION Needles: 6 dispensing, 6 aspirations, 1 cleaning
(8 step washing sequence for each cuvette)

REAGENTS TRAY Removable rack
30 bottles, 50 mL or 24 ml (up to 1500 ml total)

SAMPLES TRAY (Sphera standard) Removable tray, 60 numbered positions,
tubes of 12 – 13 mm, 5 – 7 mL / cups of 0.5-1.5 mL (cups require a metal adapter for level detection)
(Optional) Removable tray, 20 + 20 numbered positions, 20 tubes of 12 – 16 mm / 20 cups (3,5 mL type)

CUVETTE ROTOR 80 washable BIONEX(r) cuvettes REACTION CELLS which allow up to 30 000 tests per rotor
Optical path 6 mm, 210 – 350 µL reaction volume
100W heating resistance, temperature sensor, safety thermostat

OPTICAL GROUP 1 halogen lamp (6 V, 10 W) with extended UV emission 2 focusing lenses, optical glass
10-position filter disk: 8 positions provided with interference filters of 340, 405, 505, 546, 578, 600, 650, 700 nm wavelengths, 1 free position and 1 solid position for dark reading
Direct reading reaction cuvettes, 6mm optical path
±2 nm on peak wavelength, band pass of ±10 nm

PHOTOAMPLIFIER Photoelectric detector
Signal amplifier
Response range, 340 nm to 900 nm Photometric range, 0 to 2.5 Abs Linearity, ±0.5% full scale
Precision: 0.5 CV% (0.050 to 1.500 Abs)
Stability: daily reader offset, less than 1% drift per day

CONTROL Real-time multitasking microprocessor-based control Easy access to the electronics

EXTERNAL (Minimum requirements for Software v.1.0)

PIPETTING Volume: sample, 2 – 300 µl; reagent, 5-350 µL
Precision: 1.5 CV% at 2 µl; 1 CV% at 4 µL Mixing by sample needle upon dispensation

REACTION volume, 210 – 350 µL

SAMPLE In-needle dilution if allowed by method’s sample volumes DILUTION Automatic pre-dilution in a reaction cuvette, up to 1:100

TEMPERATURE Reagent refrigeration, circa 12 °C below room temperature CONTROL Reaction cells, heating unit can be set from
room temperature up to 42 °C ±0.2 ºC (108 °F ±0.5 ºF)

TYPES OF TESTS Endpoint, Biochromatic endpoint, Differential endpoint,
Differential endpoint sample blank, Fixed Time, Kinetic

TEST RUNS Random / Urgent

MEASUREMENT 125 tests/hour for double reagent run RATES 200 tests/hour in single reagent mode
Maximum incubation + reading time: 638 seconds Typical precision, endpoint 2.0 CV% / kinetic 2.0 CV% Carry-over, lower that 15 parts per million

START-UP The start-up procedure is run daily: self-test,
reader offset of optics, wash and check of all cuvettes

CALIBRATION Reagent blank subtraction, 1 to 8 standards per test method Linear: factor, linear, linear regression (standard’s repetitions)
Nonlinear (3 interpolation types): cubic-spline, poly-linear and logit-log four parameters
Free standard / control positions on all the sample plate Results can be recalculated when changing factor or curve

MAINTENANCE Procedures programmed by component life counters

PRINTING Single test, complete sample, work sheet, method and QCs REPORTS Automatic sample reports upon test completion if requested

NEEDLE Sampling needle washed internally and externally
WASHING with system solution after every operation

POWER Standard VDE removable power cord

EXTERNAL PC USB port

HOST/ LIS Ethernet LAN (samples, work list, results)
Standard ASTM ASCII protocol

WORKLIST/ For each worklist: unlimited number of samples, unlimited SAMPLES number of tests, up to 99 sheets of tests per worklist.
Sample-patient ID codes

TEST METHODS Unlimited number of methods in PC memory 40 active methods